Skills and Expertise

• Bacterial culture: Escherichia coli (12 differents strains, 6 pathogenic P2 level), Lactobacillus rhamnosus (11 differents strains).

• Biochemical assays: Western Blot (SDS-PAGE, NuPAGE; LI-COR machine), Dot blot (Filter retardation assay), Co-immunoprecipitation.

• Biomolecular techniques: PCR (cloning, screening of contaminants), qPCR.

• Cell culture: 

  • • • • Immortalized cell lines: BV2, Caco-2, COS-7, E.G7-OVA, HEK 293T, Hep G2, HeLa, IMG, Neuro-2a, PC-12, SH-SY5Y.

        • iPSC cells: human iPSC Ngn2-inducible, patient-derived Dopaminergic Neurons, iPSC-derived glutamatergic neurons (iNeurons), iPSC-derived dopaminergic neurons.

• Cell transfection: Calcium phosphate, Lipofection (Lipofectamine 2000/3000, ViaFect, FastFect), Nucleofection, PEI.

• Cell-based assays: 

  • • • • Oxidative stress readouts: CellRox Green/Red (Thermofisher), DCFDA (Abcam), Mitosox (Thermofisher), tBARS (RD Systems).

        • Cell-death readouts: CellEvent caspase3/7 (Thermofisher), Annexin V/PI (Thermofisher), LDH release (Thermofisher).

        • Quality controls: Mycoplasma detection kits (ATCC).

• Immunocytochemistry: One to four colors staining in immortalized or iPSC cell lines.

• Lentivirus: Production of 3rd generation lentivirus, infection and creation of stable cell lines.

• Microscopy: 

  • • • Confocal microscopy: LSM 770 (Carl Zeiss, fixed samples), LSM 780 (Carl Zeiss, fixed samples), Leica DMI-8 STED 3X (Leica, Fixed samples, live-cell imaging - Super resolution), Quorum Wave FX Spinning Disc (Live-cell imaging)

      • Epifluorescent microscopy: Axio observer with apotome (Carl Zeiss, live-cell imaging)

      • Light microscopy:

• Fields of expertise: Alpha-synuclein, Neurology, Microbiology, Oxidative Stress, Parkinson's disease, Protein aggregation, Protein misfolding, Endolysosomal system, Endosomes, Lysosomes.

Computer software

• Bibliographic tools: Zotero.

• Biomolecular tool: SnapGene

• FACS software: FlowJo.

• Imaging post-treatment: Adobe photoshop (Adobe), Adobe illustrator (Adobe), Powerpoint (Office), Fiji (ImageJ).

• Imaging acquisition/analysis software: 

  • Microscope software: ZEN (Zeiss), Leica Application Suite X (LAS X, Leica)

  • Deconvolution: Huygens Software - Deconvolution module (Scientific Volume Imaging)

  • Image analysis: Fiji (ImageJ), Image Studio Lite (LI-COR)

  • 3D analysis: Imaris Software - Spots and volume analyses, surface rendering (Oxford instruments)

  • Deep learning segmentation tools: WEKA, Ilastik

• Office suite: Excel, Powerpoint, Word (Office).

• Statistic software: GraphPad Prism (GraphPad Software), Excel (Office).

• Others: I'm very flexible, open-minded and a fast self-learner when I need to use and master a new software.